Document 0104
 DOCN  M9490104
 TI    Trans-activation of the 5' to 3' viral DNA strand transfer by
       nucleocapsid protein during reverse transcription of HIV1 RNA.
 DT    9411
 AU    Darlix JL; Vincent A; Gabus C; de Rocquigny H; Roques B; LaboRetro
       INSERM, Ecole Normale Superieure de Lyon, France.
 SO    C R Acad Sci III. 1993 Aug;316(8):763-71. Unique Identifier : AIDSLINE
       MED/94319978
 AB    Two DNA strand transfer reactions take place during reverse
       transcription of the retroviral genome. The first transfer, that of the
       minus-strand strong stop DNA from the 5' end of the viral RNA to the 3'
       end, has been studied in vitro with two RNAs mimicking the 5' and 3'
       regions of the HIV1 genome and with nucleocapsid protein, NCp7, and
       reverse transcriptase. The results show that NCp7 strongly activates the
       5' to 3' DNA strand transfer during reverse transcription while a basic
       peptide resembling NCp7 is inactive. Activation of the first transfer by
       several NCp7 derived peptides and the influence of the terminal
       redundancies (R) present at the 5' and 3' ends of HIV1 RNA were also
       examined. The first transfer is optimal in the presence of intact NCp7
       and necessitates R on both the 5' and 3' RNAs. Sequencing of full length
       viral DNA products reveals approximately 40% misincorporations at the
       first nucleotide beyond the transfer point. If such base
       misincorporations occur during proviral DNA synthesis with possible
       homologous recombinations it may well contribute to the high level of
       genetic variability of HIV.
 DE    Capsid/*METABOLISM  DNA, Viral/GENETICS/*METABOLISM  Gene Products,
       gag/*METABOLISM  *Genome, Viral  HIV-1/ENZYMOLOGY/*GENETICS  In Vitro
       Reverse Transcriptase/METABOLISM  Support, Non-U.S. Gov't
       *Trans-Activation (Genetics)  Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).